Practicalities and pitfalls in the molecular lab (30 min)
ISBT Education. Yanli J.
Nov 28, 2017; 203521
Topic: Molecular testing
This content is exclusively available for members.

You may become an ISBT member here.
Learning Objectives
Discussion Forum (0)
Rate & Comment (0)
After viewing this presentation, the participant will be able to:

- Understand the genetic background of D variants in the Chinese population.

- Evaluate the serological and molecular characters of DEL phenotype.

- Review the main application of molecular testing in China.
Molecular immunohematological techniques has been well applied in the transfusion medicine. Here we review the major applications for blood group genotyping in China. Its application mainly focuses on the typing of blood group antigens in complex cases, such as ABO subgroups, D variants, or the Asian specific and common antigens typing (such as the antigens expressed by the MNS hybrid glycophorins), also because of lacking of the corresponding commercial antibodies. The comprehensive antigens profile in some specific samples could be obtained by using medium or high-throughput genotyping platforms. Whereas, fetal RHD genotyping in D– pregnant women in Europe is highly relevant to avoid unnecessary antenatal RhIg, it is of comparatively less importance in the Chinese D– pregnant women because of the low probability (3-4%) to have a D– baby. In contrast, the application of routine molecular testing for the “Asia type” DEL, which having a frequency up to 30% in East Asian people serologically typed as D-negative, is more necessary to avoid unnecessary administration of antenatal RhIg, because there is increasing evidence that individuals carrying the “Asia type” DEL allele (c.1227G>A, p.Lys409Lys) do not produce alloanti-D after exposure to D+ red blood cells. The molecular methods to detect the RHD*1227A is more accurate and applicable for routine testing of “Asia type” DEL.
Code of conduct/disclaimer available in General Terms & Conditions
Anonymous User Privacy Preferences

Strictly Necessary Cookies (Always Active)

MULTILEARNING platforms and tools hereinafter referred as “MLG SOFTWARE” are provided to you as pure educational platforms/services requiring cookies to operate. In the case of the MLG SOFTWARE, cookies are essential for the Platform to function properly for the provision of education. If these cookies are disabled, a large subset of the functionality provided by the Platform will either be unavailable or cease to work as expected. The MLG SOFTWARE do not capture non-essential activities such as menu items and listings you click on or pages viewed.

Performance Cookies

Performance cookies are used to analyse how visitors use a website in order to provide a better user experience.

Google Analytics is used for user behavior tracking/reporting. Google Analytics works in parallel and independently from MLG’s features. Google Analytics relies on cookies and these cookies can be used by Google to track users across different platforms/services.

Save Settings