Practicalities and pitfalls in the molecular lab (30 min)
ISBT Education. Yanli J. Nov 28, 2017; 203521
Topic: Molecular testing
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Learning Objectives
Abstract
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After viewing this presentation, the participant will be able to:

- Understand the genetic background of D variants in the Chinese population.

- Evaluate the serological and molecular characters of DEL phenotype.

- Review the main application of molecular testing in China.
Molecular immunohematological techniques has been well applied in the transfusion medicine. Here we review the major applications for blood group genotyping in China. Its application mainly focuses on the typing of blood group antigens in complex cases, such as ABO subgroups, D variants, or the Asian specific and common antigens typing (such as the antigens expressed by the MNS hybrid glycophorins), also because of lacking of the corresponding commercial antibodies. The comprehensive antigens profile in some specific samples could be obtained by using medium or high-throughput genotyping platforms. Whereas, fetal RHD genotyping in D– pregnant women in Europe is highly relevant to avoid unnecessary antenatal RhIg, it is of comparatively less importance in the Chinese D– pregnant women because of the low probability (3-4%) to have a D– baby. In contrast, the application of routine molecular testing for the “Asia type” DEL, which having a frequency up to 30% in East Asian people serologically typed as D-negative, is more necessary to avoid unnecessary administration of antenatal RhIg, because there is increasing evidence that individuals carrying the “Asia type” DEL allele (c.1227G>A, p.Lys409Lys) do not produce alloanti-D after exposure to D+ red blood cells. The molecular methods to detect the RHD*1227A is more accurate and applicable for routine testing of “Asia type” DEL.
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